Table 1.
Experimental and theoretical binding properties of Ca2+-CaM-BIMs complexes.
| Experimental Studies | Docking Studies | |||||
|---|---|---|---|---|---|---|
| Kd (nM) | Stoichiometry | Ki (nM) | ||||
| Site I | Site II | Site III | Site IV | |||
| Ca2+-CaM -BIM-II | 257.8 ± 5.5 | 2.5 ± 0.2 | 109.79 | 161.58 | - | - |
| Ca2+-CaM -BIM-IV | 223.8 ± 3.7 | 3.7 ± 0.2 | 17.10 | 3390 | 7450 | - |
| Ca2+-CaM -BIM-VII | 186.2 ± 4.1 | 4.4 ± 0.1 | 2.14 | 26.95 | 52.43 | 263.87 |
| Ca2+-CaM -BIM-X | 205.2 ± 3.8 | 4.3 ± 0.1 | 20.36 | 59.39 | 71.65 | 217.1 |
| Ca2+-CaM -BIM-XI | 239.0 ± 5.0 | 3.4 ± 0.1 | 9.66 | 55.96 | 75.12 | 191.71 |
| Ca2+-CaM -CPZ | 492.2 ± 4.6 | 3.4 ± 0.1 | 715.65 | 1169 | 1640 | - |
| Ca2+-CaM -TFP | 532.7 ± 74.2 1 | 1.6 ± 0.2 | 384 | 707 | 959 | 976 |
1 This data was taken from previous experiments of the same working group [33].