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. 2022 Nov 3;23(21):13482. doi: 10.3390/ijms232113482

Figure 5.

Figure 5

Effects of GEN and miR-221/222 family on C2C12 myoblast differentiation. (A) MyHC immunofluorescence staining of C2C12 myotubes after different treatments. (B) The fusion rate of C2C12 myotubes after different treatments. (C,D) Expression of miR-222 (C) and miR-221 (D) in C2C12 myotubes after different treatments. (E) The binding sites prediction of MyoG/Tnnc1 and miR-221/222 family. (F,G) Fluorescence intensity of the dual-luciferase reporter assay system for the potential binding sites of miR-221/222 family and MyoG (F)/Tnnc1 (G). (H,I) Expression of MyoG (H) and Tnnc1 (I) in C2C12 myotubes after different treatments. n = 3. * p < 0.05, compared with the NC group, # p < 0.05, compared with 222 M group, $ p < 0.05, compared with 221 M group.