Table 6.
Effect of selenium nanoparticles–MOS supplementation on the histomorphometric parameters in duodenum, jejunum and ileum broilers chickens reared under higher stocking density.
| Parameters | Control (NSD) |
Control (HSD) |
Se-0.15 + HSD |
MOS + HSD |
Se–0.15–MOS + HSD | SeNP + HSD |
SeNP–MOS + HSD | Pooled SEM |
p-Value |
|---|---|---|---|---|---|---|---|---|---|
| Duodenum, 21st day data | |||||||||
| Villus height (µm) | 1137 a | 1042 b | 1115 ab | 1124 ab | 1123 ab | 1142 a | 1147 a | 8.019 | 0.007 |
| Villus width (µm) | 101.04 | 93.31 | 92.61 | 99.42 | 99.04 | 99.45 | 96.25 | 3.168 | 0.320 |
| Villus surface area (µm2) | 361,475 a | 304,792 b | 324,121 ab | 352,046 ab | 348,291 ab | 356,802 a | 347,095 ab | 4270.17 | 0.007 |
| Crypt depth (µm) | 154.94 | 160.75 | 160.08 | 158.52 | 156.44 | 160.42 | 151.37 | 1.489 | 0.732 |
| VH:CD | 7.42 ab | 6.54 b | 7.06 ab | 7.16 ab | 7.25 ab | 7.14 ab | 7.67 a | 0.247 | 0.011 |
| Duodenum, 42nd day data | |||||||||
| Villus height (µm) |
1650 a | 1317 d | 1338 cd | 1366 cd | 1425 bc | 1429 bc | 1495 b | 14.29 | 0.000 |
| Villus width (µm) |
170.65 | 158.90 | 158.77 | 158.92 | 157.49 | 155.86 | 158.27 | 1.445 | 0.275 |
| Villus surface area (µm2) | 884,642 a | 657,264 | 665,588 c | 682,652 bc | 706,649 bc | 696,825 bc | 743,346 b | 10,163.53 | 0.000 |
| Crypt depth (µm) |
174.88 bc | 197.97 a | 178.33 bc | 175.67 bc | 177.30 bc | 171.37 bc | 164.84 c | 1.778 | 0.000 |
| VH:CD | 9.53 a | 6.81 e | 7.56 cde | 7.84 cd | 8.14 cd | 8.38 bc | 9.12 ab | 0.245 | 0.000 |
| Jejunum, 21st day data | |||||||||
| Villus height (µm) | 926 | 877 | 872 | 893 | 884 | 887 | 911 | 4.657 | 0.067 |
| Villus width (µm) | 82.48 | 79.40 | 79.95 | 83.22 | 80.27 | 80.27 | 82.78 | 0.733 | 0.851 |
| Villus surface area (µm2) | 240,439 | 218,984 | 218,797 | 233,088 | 222,755 | 223,569 | 237,322 | 2399.13 | 0.162 |
| Crypt depth (µm) | 126.19 | 123.16 | 124.18 | 130.23 | 136.67 | 132.95 | 130.76 | 1.551 | 0.231 |
| VH:CD | 7.47 | 7.22 | 7.10 | 7.94 | 6.50 | 6.77 | 7.04 | 0.182 | 0.648 |
| Jejunum, 42nd day data | |||||||||
| Villus height (µm) | 1148 a | 933 c | 954 c | 975 c | 1025 bc | 958 c | 1066 ab | 12.09 | 0.000 |
| Villus width (µm) | 110.27 | 107.04 | 99.60 | 100.38 | 102.81 | 106.06 | 104.74 | 1.483 | 0.481 |
| Villus surface area (µm2) | 398,380 a | 314,717 b | 300,175 b | 307,284 b | 330,606 b | 320,809 b | 351,421 ab | 6682.17 | 0.001 |
| Crypt depth (µm) | 163.70 | 165.51 | 161.20 | 162.35 | 160.27 | 158.37 | 160.45 | 1.278 | 0.763 |
| VH:CD | 7.08 a | 5.69 d | 5.96 cd | 6.03 bcd | 6.43 abc | 6.10 bcd | 6.70 ab | 0.087 | 0.000 |
| Ileum, 21st day data | |||||||||
| Villus height (µm) | 694 | 673 | 676 | 666 | 674 | 677 | 680 | 2.552 | 0.211 |
| Villus width (µm) | 103.01 | 100.42 | 95.2944 | 97.87 | 103.39 | 106.47 | 106.71 | 1.251 | 0.168 |
| Villus surface area (µm2) | 225,440 | 212,342 | 202,381 | 204,697 | 219,426 | 226,493 | 227,966 | 2896.18 | 0.094 |
| Crypt depth (µm) | 83.69 | 90.24 | 88.14 | 88.78 | 87.54 | 88.75 | 88.57 | 0.749 | 0.590 |
| VH:CD | 8.34 | 7.53 | 7.74 | 7.57 | 7.78 | 7.73 | 7.75 | 0.072 | 0.156 |
| Ileum, 42nd day data | |||||||||
| Villus height (µm) | 801 | 748 | 762 | 757 | 769 | 755 | 799 | 5.948 | 0.174 |
| Villus width (µm) | 163.61 | 147.78 | 153.28 | 151.97 | 158.07 | 152.41 | 155.35 | 1.821 | 0.451 |
| Villus surface area (µm2) | 411,334 a | 347,431 b | 366,548 b | 361,416 b | 381,458 ab | 361,616 b | 389,802 ab | 5236.46 | 0.044 |
| Crypt depth (µm) | 169.46 | 174.62 | 170.91 | 169.44 | 163.94 | 166.67 | 164.40 | 1.060 | 0.102 |
| VH:CD | 4.75 ab | 4.35 c | 4.39 bc | 4.52 abc | 4.72 abc | 4.55 abc | 4.89 a | 0.044 | 0.012 |
a–e within the same row means that those with different superscripts are significantly different (p < 0.05). Values represent means of eight replicates. NSD: normal stocking density (10 birds/m2), HSD: high stocking density (16 birds/m2), Se: selenium, SeNP: selenium nanoparticles, MOS: mannan oligosaccharide, VH:CD: villus height to crypt depth ratio.