Skip to main content
. 2022 Nov 5;23(21):13578. doi: 10.3390/ijms232113578

Figure 1.

Figure 1

Myeloid SIRT1 is essential for host protection against T. gondii infection in primary macrophages and mice. (A–C) BMDMs from mSirt1+/+ and mSirt1−/− mice were infected with GFP-conjugated T. gondii RH strain (MOI = 1) for the indicated periods. Cells were fixed and stained with Texas Red®-X phalloidin for F-actin in the cytoskeleton (red) and DAPI for nuclei (blue), respectively. (A) Fluorescent images showing the number of intracellular T. gondii. (B,C) Number of T. gondii RH-infected cells (for B) and T. gondii RH per vacuole (for C) were quantified. Scale bar = 25 µm (D) BMDMs from mSirt1+/+ and mSirt1−/− mice were infected with T. gondii RH strain (MOI = 1) for the indicated time periods. TP3 protein expression (top) and Sag1 mRNA expression (bottom) was examined by immunoblot and qPCR analysis, respectively. (E,F) mSirt1+/+ and mSirt1−/− mice (n = 5 per group) were infected with 40 cysts of the ME49 strain (i.p. injection) for 3 weeks. (E) The number of cysts in the brain was counted under a microscope. (F) Sag1 mRNA expression in brain homogenates was evaluated by real-time qPCR analysis. Data are representative of three independent experiments and are presented as means ± SD (for B–D) or ± SEM (for F). * p < 0.05, ** p < 0.01, *** p < 0.001, compared with control cultures or control mice infected with T. gondii (two-tailed Student’s t-test). Tg, Toxoplasma gondii.