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. 2022 Oct 28;23(21):13134. doi: 10.3390/ijms232113134

Figure 5.

Figure 5

The effects of heat or TM treatment on the expression of the AtDjC5 gene by Q-PCR. (A) Time course of heat-induced AtDjC5 expression. Ten-day-old seedlings grown at 22 °C were heated at 37 °C for the length of time indicated. The level of AtDjC5 in the unheated seedlings was normalized to 1. (B) The effect of TM treatment on AtDjC5 expression. Ten-day-old seedlings grown at 22 °C were treated with 5 μg/mL TM for 2 h. The level of AtDjC5 in the nontreated seedlings was normalized to 1. Q-PCR was performed with AtDjC5-specific primers (Table S1). Actin was used as an internal control. Data are the mean ± SD from three independent experiments.