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. 2022 Oct 25;11(21):3357. doi: 10.3390/cells11213357

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Analysis of ZO-1 expression (green) by confocal laser scanning microscopy (CLSM) in different (co)-culture conditions after 21 days of culture. Caco-2 mono-culture; Caco-2/HT29-MTX co-culture; Caco-2/Raji B co-culture; Caco-2/HT29-MTX co-culture; Caco-2/HT29-MTX/Raji B tri-culture. Nuclei were counterstained with DAPI (blue). Bars, 20 µm.