Figure 11.

SiO₂ nanobeads (red) internalization in Caco-2 monoculture, Caco-2/HT29-MTX co-culture, Caco-2/Raji B co-culture, and Caco-2/HT29-MTX/Raji B tri-culture on 3 µm pore insert. Cells were counterstained with DAPI (blue) and ZO-1 (green). Representative 3D reconstructions (Z-stacks) by CLSM are reported. 50–70 Z-slices were acquired every 0.20 µm covering the total height of the cell cultures on inserts. Left panels show xy Z-stack projections, while right panels show xz Z-stack projections; the upper and the lower surface of inserts are indicated. Scale bars are 20 and 10 µm, as indicated.