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. 2022 Oct 24;23(21):12796. doi: 10.3390/ijms232112796

Figure 1.

Figure 1

Characterization of the contribution of the TMDs of iR1 and iR2 to the substrate selectivity of stimulated ADAM17. Ectodomain shedding assays were performed in iR1/2−/− mEFs co-transfected with iR1 or iR2 or chimeras between iR1 and iR2 (iR1, iR2, iR21,2/iR13–7, iR11,2/iR23–7, iR11–6/iR27, iR21–6/iR17, see Supplementary Figure S1 for details) and TGFα (A), EREG (B) or KL2 (C). In the diagrams below each graph, components of iR1 are shown in red, and components of iR2 are shown in blue. Constitutive (no treatment) and PMA-stimulated shedding (+25 ng/mL PMA) for each variable were measured after 1 h of stimulation. Results are shown as mean ± SEM (n = 3 for (A,C), n = 6 for (B)), * indicates p ≤ 0.005 in an unpaired t-test between the untreated and PMA-treated condition for a given sample.