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. 2022 Oct 24;23(21):12796. doi: 10.3390/ijms232112796

Figure 3.

Figure 3

Point mutations in the TMD7 of iR1 allow it to support limited stimulation of EREG shedding. (A) Schematic representation of the TMD7 of iR1 and iR2 as an α-helix. Point mutations were designed to change amino acid residues on the same face of the TMD7 α−helix (red circles, iR1-F807S, labeled as iR1-F8S), iR1-L815F (labeled as iR1-L16F)). Amino acid residues with large side chains (W, Y, F) are shown in green letters. The position of iR2-S808 that is important for the selectivity of iR2 (see Figure 2F) and the corresponding iR1-F807 are marked in orange (B,C) Ectodomain shedding assays were performed in iR1/2−/− mEFs co-transfected with iR1 carrying the point mutations indicated in (A) and with TGFα (B) or EREG (C). Results are shown as mean ± SEM (n = 3), * denotes p ≤ 0.005 between the unstimulated and PMA (+) condition for a given sample.