TABLE 3.
Characteristics of TLF1 and TLF2
| Characteristic | TLF1 | TLF2 |
|---|---|---|
| Mol wt | 500,000 | 1,000,000 |
| % Lipid | 40 | <1 |
| Hpr | + | + |
| ApoA-I | + | + |
| IgM | − | + |
| Paraoxonase/arylesterasea | ∼20 ng/2 μg | <20 ng/4 μg |
| ApoA-IIb | Trace detected | Not detected |
| Inhibited by Hp | + | − |
| Hbc | 22 ng/μg | <1 ng/1.8 μg |
Paraoxonase, 0.000012 U/μg of HDL; arylesterase, 0.00013 U/μg of HDL; no activity was detected in 1 μg of TLF1 or 1.8 μg of TLF2. Paraoxonase protein was not detected in TLF2 (4 μg) by antiparaoxonase MAb F41F2-K, which has a limit of detection of 20 ng. Paraoxonase protein was detected in TLF1 at a level is estimated to be 10-fold less than that detected in HDL; 1 μg of HDL contains ∼70 ng of paraoxonase.
Not detected by silver stain or Coomassie blue stain; trace amounts were detected by ECL Western blotting.
Limit of detection of 1 ng by immunoblot with polyclonal goat anti-human Hb (Sigma). Hb detected in TLF1 cofractionates with Hp but not Hpr or lytic activity (∼500 kDa) in the final size fraction of purification, which may represent contaminating Hp-Hb complexes of ∼150 kDa.