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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: Nat Protoc. 2022 Jan 12;17(2):476–512. doi: 10.1038/s41596-021-00653-8
Component Amount per reaction (μL) Final concentration
JumpStart, 2X 25 1X
NGS_TruSeq_P5 index primer, 5 μM 1.25 0.125 μM
NGS_TruSeq_P7 index primer, 5 μM 1.25 0.125 μM
PCR1 product from Step 39 OR dialout PCR from Step 8 1
UltraPure water up to 50 μL
Total 50