| Component | Amount per reaction (μL) | Final concentration |
|---|---|---|
| JumpStart, 2X | 25 | 1X |
| NGS_TruSeq_P5 index primer, 5 μM | 1.25 | 0.125 μM |
| NGS_TruSeq_P7 index primer, 5 μM | 1.25 | 0.125 μM |
| PCR1 product from Step 39 OR dialout PCR from Step 8 | 1 | |
| UltraPure water | up to 50 μL | |
| Total | 50 |
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