| Component | Amount per well of 6-well plate |
|---|---|
| Opti-MEM | 166 μL |
| pMD2.G (lentiviral helper plasmid) | 704 ng |
| psPAX2 (lentiviral helper plasmid) | 1056 ng |
| sgRNA plasmid library from Step 38 | 1408 ng |
| P3000 reagent | 12 μL |
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