| Component | Amount per reaction (μL) | Final concentration |
|---|---|---|
| JumpStart, 2X | 25 | 1X |
| NGS_CROPseq-puro_P5 primer, 10 μM | 0.75 | 0.15 μM |
| NGS_CROPseq-puro_P7 primer, 10 μM | 0.75 | 0.15 μM |
| Cell lysate from Step 81 | 12.5 ul | 2 ng/μL |
| UltraPure water | up to 50 μL | |
| Total | 50 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.