| Component | Amount per well (μl) | Final |
|---|---|---|
| UltraPure water | 533 | |
| RevertAid RT buffer, 5X | 150 | 1X |
| dNTPs, 10mM each | 19 | 250μM |
| LNA-modified RT primer, 100μM; see Supplementary Table 4 for oligo sequence | 7.5 | 1μM |
| BSA, molecular biology grade, 20 mg/mL | 7.5 | 0.2 mg/mL |
| Ribolock RNase inhibitor, 40 units/μL | 15 | 0.8 units/μL |
| RevertAid H minus Reverse Transcriptase, 200 units/μL | 18 | 4.8 units/μL |
| Total volume | 750 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.