| Component | Amount per well (μl) | Final |
|---|---|---|
| UltraPure water | 422 | |
| Ampligase buffer, 10X | 60 | 1X |
| dNTPs, 10μM each | 3 | 50nM |
| Padlock probe, 100μM; see Supplementary Table 4 for oligo sequence | 0.6 | 0.1μM |
| BSA, molecular biology grade, 20 mg/mL | 6 | 0.2 mg/mL |
| RNase H, 5 units/μL | 48 | 0.4 units/μL |
| TaqIT DNA polymerase, 50 units/μL | 0.24 | 0.02 units/μL |
| Ampligase, 5 units/μL | 60 | 0.5 units/μL |
| Total volume | 600 |
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