| Component | Amount per well (μL) | Final |
|---|---|---|
| UltraPure water | 399 | |
| Glycerol, 50% | 60 | 5% |
| Phi29 buffer, 10X | 60 | 1X |
| dNTPs, 10mM each | 15 | 250 μM |
| BSA, molecular biology grade, 20 mg/mL | 6 | 0.2 mg/mL |
| Phi29 DNA polymerase, 10 units/μL | 60 | 1 unit/μL |
| Total volume | 600 |
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