Table 1 |.

Recommended microscope components

Component	Recommended Options	Description
Microscope body	Nikon Ti2	Large field-of-view and fast stage reduce acquisition time; note that custom sizing of filters may be required
Control software	Micro-Manager	Micro-Manager is open source and freely available; however, NIS Elements allows more flexibility, such as easier definition of imaging sites, improved alignment and autofocusing options, and saving of imaging workflows using the JOBS module
	NIS Elements with JOBS module
Camera	Iris 9 (Teledyne Photometrics); Sensor: 12.58 × 12.58 mm; 2960 × 2960 pixels	The goal is to have a sensor area large enough to take advantage of the full field-of-view of the microscope. For cameras with smaller sensor areas, a 0.7X relay lens coupler can be used (e.g., Nikon, cat. no. MQD42075), although this decreases resolution and may result in vignetting.
	ORCA-Flash4.0 v3 (Hamamatsu Photonics); Sensor: 13.31 × 13.31 mm; 2048 × 2048 pixels
	ORCA-Fusion (Hamamatsu Photonics); Sensor: 14.98 × 14.98 mm; 2304 × 2304 pixels
Objective Lenses	10X air NA 0.45 (e.g., Nikon, cat. no. MRD00105)	For SBS and some phenotype image acquisition
	20X air NA 0.75 (e.g., Nikon, cat. No. MRD00205)	Optional for phenotype image acquisition