| Component | Amount per reaction (μL) | Final concentration |
|---|---|---|
| Oligo pool from Step 4 | 1 | 0.2 ng/μL |
| Forward dialout primer, 100 uM | 0.15 | 0.3 μM |
| Reverse dialout primer, 100 uM | 0.15 | 0.3 μM |
| KAPA HiFi HotStart ReadyMix, 2X | 25 | 1X |
| UltraPure water | 23.7 | |
| Total | 50 |
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