| Component | Amount per reaction (μL) | Final concentration |
|---|---|---|
| Library plasmid backbone | 1 | 50 ng/μL |
| FastDigest Esp3I, 1 unit/μL | 1 | 0.05 units/μL |
| FastDigest buffer, 10X | 2 | 1X |
| UltraPure water | 16 | |
| Total | 20 |
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