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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: Nat Protoc. 2022 Jan 12;17(2):476–512. doi: 10.1038/s41596-021-00653-8
Component Amount per reaction (μL) Final concentration
JumpStart, 2X 25 1X
NGS_CROPseq-puro_P5 primer, 10 μM 0.75 0.15 μM
NGS_CROPseq-puro_P7 primer, 10 μM 0.75 0.15 μM
Pooled sgRNA library plasmid from Step 38 100 ng 2 ng/μL
UltraPure water up to 50 μL
Total 50