| Component | Amount per reaction (μL) | Final concentration |
|---|---|---|
| JumpStart, 2X | 25 | 1X |
| NGS_CROPseq-puro_P5 primer, 10 μM | 0.75 | 0.15 μM |
| NGS_CROPseq-puro_P7 primer, 10 μM | 0.75 | 0.15 μM |
| Pooled sgRNA library plasmid from Step 38 | 100 ng | 2 ng/μL |
| UltraPure water | up to 50 μL | |
| Total | 50 |
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