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. 2022 Nov 4;23(21):13537. doi: 10.3390/ijms232113537

Table 1.

Significant protein spot differences, from Twi vs. Het, Het vs. WT, and Twi vs. WT comparisons, identified by mass spectrometry.

a Spot
N.		 b Protein Name c UniProtKB Accession Number d MetaCore
Name		 e Mascot Search Results f Fold Change
Score Expected Matched Peptides/
Detected Peptides		 Sequence
Coverage
%		 Twi/Het Het/WT Twi/WT
1 Ubiquitin carboxyl-terminal hydrolase 5 P56399 USP5 144 6.8 × 10−11 14/18 15 4.05 ¥ 0.22 ¥ 0.86 NS
2 Transitional endoplasmic reticulum ATPase Q01853 VCP 226 4.3 × 10−19 22/27 25 2.52 ¥ 0.59 NS 1.48 NS
3 Aconitate hydratase, mitochondrial Q99KI0 ACON 188 2.7 × 10−15 13/14 25 0.70 NS 2.89 # 2.02 NS
4 Aconitate hydratase, mitochondrial Q99KI0 ACON 100 1.7 × 10−6 9/15 16 2.32 ¥ 1.33 NS 3.07 #
6 Aconitate hydratase, mitochondrial Q99KI0 ACON 258 2.7 × 10−22 20/22 31 2.58 ¥ 0.72 NS 1.84 NS
7 Albumin P07724 Albumin 414 6.8 × 10−38 29/32 52 2.66 NS 0.26 ¥ 0.67 NS
9 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial Q8BMF4 ODP2 119 2.2 × 10−8 10/14 20 1.34 NS 1.50 NS 2 ¥
10 Dihydropyrimidinase-related protein 2 O08553 CRMP2 233 8.6 × 10−20 17/23 43 2.00 # 0.73 NS 1.45 ¥
11 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial P53395 BCKD-E2 127 3.4 × 10−9 12/18 17 0.32 # 1.69 NS 0.54 NS
12 2′,3′-cyclic-nucleotide 3′-phosphodiesterase P16330 CNP1 210 1.7 × 10−17 16/25 41 2.47 # 0.47 # 1.14 NS
13 Vimentin P20152 Vimentin 98 3.00 × 10−6 7/9 19 12.75 ¥ 0.46 NS 5.77 ¥
14 ATP synthase subunit beta, mitochondrial P56480 ATP5B 171 1.4 × 10−13 13/17 32 3.98 ¥ 0.33 ¥ 1.29 NS
15 Protein NDRG1 Q62433 NDRG1 101 1.4 × 10−6 8/9 24 0.91 NS 2.61 # 2.35 ¥
16 NSFL1 cofactor p47 Q9CZ44 p47 118 2.7 × 10−8 8/12 29 3.74 ¥ 0.63 NS 2.33 ¥
17 Tropomodulin-2 Q9JKK7 Tropomodulin-2 100 1.7 × 10−6 9/19 33 3.06 ¥ 0.28 ¥ 0.86 NS
18 60S acidic ribosomal protein P0 P14869 RPLP0 194 6.8 × 10−16 12/15 47 0.52 NS 2.01 ¥ 1.03 NS
19 Beta-soluble NSF attachment protein P28663 NAPB 227 3.4 × 10−19 18/22 60 1.67 NS 1.60 NS 2.67 ¥
20 Creatine kinase B-type Q04447 KCRB 127 3.4 × 10−9 7/7 21 2.72 ¥ 0.67 NS 1.82 NS
22 Receptor of activated protein C kinase 1 P68040 RACK1 189 2.2 × 10−15 13/23 59 3.14 # 0.42 ¥ 1.30 NS
23 Protein-L-isoaspartate(D-aspartate) O-methyltransferase P23506 PIMT 173 8.6 × 10−14 12/15 42 0.39 ¥ 1.71 NS 0.66 NS
24 Translationally-controlled tumor protein P63028 TPT1 136 4.3 × 10−10 10/18 44 1.05 NS 2.7 ¥ 2.83 #
25 Transgelin-3 Q9R1Q8 Transgelin-3 159 2.2 × 10−12 16/30 56 0.53 ¥ 2.24 ¥ 1.19 NS
27 Myelin basic protein P04370 Myelin basic protein 151 1.4 × 10−11 10/13 30 0.59 ¥ 2.12 # 1.25 NS

a Spot numbers match those used in Figure 1 to indicate protein spot differences; b UniProtKB protein name; c UniProtKB accession number; d MetaCore name; e Mascot search results: number of matched peptides, sequence coverage, and score; f Fold changes calculated on %Vol values computed for identified spot differences matched in inter-class analysis. Protein differences were considered significant according to a FC ≥ 2 and a p ≤ 0.01 (#) or 0.01 < p ≤ 0.05 (¥). NS: not significant.