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. 2022 Oct 31;23(21):13251. doi: 10.3390/ijms232113251

Figure 1.

Figure 1

An iHFC (high-fat/cholesterol/cholate-based) diet induces steatohepatitis in the liver of Tsumura-Suzuki non-obese (TSNO) mice. (A) Representative photos of the livers from TSNO mice fed with the normal diet (ND) or iHFC diet for the indicated time periods. Scale bars, 1 cm. (B) Liver weights of TSNO mice fed with the ND or iHFC diet for the indicated time periods (n = 6 per group). (C) Left, plasma alanine aminotransferase (ALT) levels were measured for TSNO mice fed with the ND or iHFC diet for the indicated time periods (n = 6 per group). Right, plasma ALT levels per liver weight (g) were also calculated (n = 6 per group). (D) Representative images of hematoxylin and eosin-stained sections of the livers from TSNO mice fed with the ND or iHFC diet for the indicated time periods. Scale bars, 100 μm. (E) RT-qPCR of TNF-α (Tnf), iNOS (Nos2), CCL2 (Ccl2), CD11c (Itgax), and IL-1β (Il1b) mRNA in the livers from TSNO mice fed with the ND or iHFC diet for the indicated time periods (n = 3 per group). Data are shown as means ± SD. * p < 0.05, *** p < 0.001. Statistical significance was evaluated by 2-way ANOVA followed by post hoc Sidak test.