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. 2022 Oct 31;23(21):13251. doi: 10.3390/ijms232113251

Figure 5.

Figure 5

The iHFC diet accumulates two types of recruited macrophage subsets, CD11c+/Ly6C− and CD11c−/Ly6C+ cells. (A) Representative flow cytometry data of CD11c and Ly6C expression on F4/80+ non-parenchymal cells excluding KCs of the livers from TSNO mice on the ND or iHFC diet for the indicated time periods. (B) Percentage and cell number of CD11c+/Ly6C− or CD11c−/Ly6C+ were determined by flow cytometry analysis done in (A) (n = 3 or 4 per group). (C) Representative histological images of CD11c and Ly6C immunostaining of the livers from TSNO mice on the ND or iHFC diet for the indicated time periods. Scale bars, 100 μm. (D) Representative magnified images of CD11c and Ly6C immunostaining of the livers from TSNO mice on the iHFC diet for 24 weeks. Scale bars, 50 μm. (E) Positive areas for CD11c or Ly6C in the livers from TSNO mice on the iHFC diet for the indicated time periods were measured using ImageJ software. Data are shown as means ± SD. ** p < 0.01, *** p < 0.001. Statistical significance was evaluated by two-way ANOVA followed by post hoc Sidak test.