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. 2022 Nov 1;23(21):13364. doi: 10.3390/ijms232113364

Figure 2.

Figure 2

The effect of THGP on caspase-1 expression in THP-1 cells. (a–c) The effect of THGP on the expression of Caspase-1 in THP-1 cells treated with or without 10 μg/mL LPS and 1 mM ATP was investigated by western blotting. β-Actin was used as an internal standard to quantify the expres-sion of each protein. (d) Caspase-1 activity in THP-1 cells treated with 10 μg/mL LPS, 1 mM ATP and/or 5 mM THGP was measured using a Caspase-Glo® 1 Inflammasome Assay Kit. (e) The effect of THGP on the mRNA expression of IL-1β in THP-1 cells treated with 10 μg/ml LPS and 5 mM THGP was investigated by real-time RT–PCR. The mean and S.D. are shown. n = 6. * p < 0.01, ** p < 0.05.