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. 2022 Nov 1;23(21):13364. doi: 10.3390/ijms232113364

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Suppressive effect of THGP on pyroptosis induced by LPS/ATP. (a) The viability of THP-1 cells stimulated with 10 μg/mL LPS and 1 mM ATP was investigated using the MTS assay. (b) Cytotoxicity following LPS/ATP stimulation was investigated using the LDH assay. (c,d) The number of dead cells was determined by Hoechst/PI staining. Red and blue cells are dead (PI) and live cells (Hoechst), respectively (Scale bar: 100 μm). (e,f) The expression of caspase-3 was measured by western blotting. β-Actin was used as an internal standard to quantify the expression of each protein. The mean and S.D. are shown. n = 6. ** p < 0.01.