TABLE 1.

Nitrite production is dependent on IFN-γ and TNF-α production by CD40L-activated SC^a

MAb added to CD40L-activated SC	Nitrite production (μM) by macrophages
Isotype control	60.7 ± 5.1
Anti-IFN-γ	11.7 ± 5.7***
Anti-TNF-α	12.9 ± 2.6***

^aSC were activated with 3T3-CD40L transfectants in the presence of anti-IFN-γ or anti-TNF-α MAb. Supernatants were harvested after 48 h and transferred to MPM cultures at the time of T. cruzi infection. Nitrite levels were measured after 24 h. Data are means ± standard deviations of three independent experiments performed in duplicate. ∗∗∗, P < 0.001 compared to isotype control (Student’s t test).