Figure 1.

Quantification of H₂O₂ in the cell culture medium and analysis of the formation of intracellular ROS. (A) Quantification of H₂O₂ in the cell culture medium treated with different concentrations of H₂O₂ and GOx for 1 and 24 h. (B) Intracellular ROS levels were quantified after treatment with different concentrations of H₂O₂ and (C) GOx for 50 min (data presented as bar graphs for H₂O₂ concentration measured from the fluorescent intensity with Amplex UltraRed, and ROS measured from relative fluorescence intensity normalized to cell count with CM-H₂DCFDA ROS indicator, n = 5, mean and standard deviation of the mean, significance compared to the respective control, two-way ANOVA with Dunnett’s multiple comparison test, * p < 0.05, ** p < 0.001).