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. 2022 Nov 3;23(21):13435. doi: 10.3390/ijms232113435

Figure 4.

Figure 4

Inflammation-related signaling upon treatment of adMSC with 0.05, 0.1 mM H2O2, and 2.5 mU/mL GOx. (A) Quantification of IL-8 releases into the supernatant (analysis by enzyme-linked immunosorbent assay, ELISA). (B) Quantification of nuclear translocation of NF-ҡB normalized to cell number (analysis of nuclei fluorescence by Athena software). (C) Intracellular Ca2+ level detection with the fluo-3/AM calcium indicator (analysis by flow cytometry). (D) Depiction of differentially released adipokines with ‘Human Adipokine Array Kit’ (pixel intensities were quantified by densitometry using the Image Lab 3.0.1 software, n = 5, significance compared to the control, two-way ANOVA with Dunnett’s multiple comparison test, * p < 0.05, ** p < 0.001).