Figure 5.

Migratory capacity of adMSC tested by the scratch assay. (A) Fluorescent depiction of adMSC preconditioned with H₂O₂ and GOx for 24 h in the scratch area (referred to as setting 1: cell staining was with cell tracking red dye, scale bar: 50 µm), and imaging at an initial time point, 0 h, and 20 h after scratching. (B) Quantification of wound closure after cells were preconditioned 24 h with H₂O₂ and GOx before scratch, and no incubation with H₂O₂ and GOx during migration (setting one); (C) adMSC preconditioned 2 h before scratch and exposed to H₂O₂ and GOx during migration (setting two). % wound closure normalized to the control; (n = 5, significance compared to the control, two-way ANOVA with Dunnett’s multiple comparison test, * p < 0.05, ** p < 0.001).