Figure 6.

Adipogenic differentiation in oxidatively stressed adMSC after 14 days. (A) Quantification of relative cell number in adipogenic stimulated (AS) adMSC (quantified with crystal violet staining) and (B) metabolic activity (quantified by MTS conversion assay related to the cell amount). (C) Fluorescent depiction of accumulated lipid in unstimulated (US) control cultures, adipogenic stimulated control cultures, and adipogenically stimulated and oxidatively stressed adMSC treated with 0.01 and 0.05 mM H₂O₂, and 0.1 and 5 mU/mL GOx, respectively; (nuclei stained with Hoechst H33342, lipid accumulation stained with Bodipy, representative images, scale bar: 50 µm). (D) Quantification of fluorescence intensity of the lipid staining after 14 days of stimulation and repeated treatments with different concentrations of H₂O₂ and GOx (normalized values to unstimulated control; n = 5, significance compared to the stimulated control, two-way ANOVA with Dunnett’s multiple comparison test, * p < 0.05).