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RT-qPCRs were performed on mRNAs prepared from ECs transfected for 48 h with control siRNA (CTRL siRNA, solid bars) or PTX3 siRNA (diagonal bars). EPHA2 (a) and ENG (b) mRNAs were normalized to the housekeeping gene GAPDH. (a), ** p < 0.001, n = 6. (b) **** p < 0.0001, n = 6.
