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The effect of silencing PTX3 on the inflammatory profile of ECs. RT-qPCRs were performed on mRNAs prepared from ECs transfected for 48 h with control siRNA (CTRL siRNA, solid bars) or PTX3 siRNA (diagonal bars). In (a), COX-2 mRNA was normalized to the housekeeping gene 18S; in (b–d), IL-1β, IL-6 and MCP-1 mRNAs were normalized to GAPDH. (a), *** p < 0.001, n = 4. (b) * p < 0.05, n = 3. (c) p = 0.143, n = 3. (d) * p < 0.05, n = 4.
