Figure 1.

Phylogenetic and sequence analyses of NKLnc. (A) Phylogenetic analysis of NKLnc. The values at the forks indicate the percentage of trees in which this grouping occurred after bootstrapping (1000 replicates; shown only when the values were >50%). Scale bar, the number of substitutions per base. (B) Sequence alignment of fish NKL homologues. The residues that are >50% identical are shaded, with identical residues shaded in dark blue. The alignment gaps are indicated with “-”. The predicted cleavage site for the signal peptide is indicated with “↓”. The conserved six cysteine residues are marked with “★”. The cysteine residues that form a disulfide bond are linked by a blue line.