The damaging effect of P35 on bacterial structure and DNA. (A–C) Vibrio harveyi was incubated with P35, the negative control peptide (NCP), or PBS for 2 h and then subjected to PI staining (A), scanning electron microscopy (B), and transmission electron microscopy (C). Scale bars, 30 μm (A), 1 μm (B), and 200 nm (C). (D) V. harveyi genomic DNA was incubated with different concentrations of P35 or with control peptide for 1 h and then subjected to electrophoresis. (E) V. harveyi was incubated with P35 for 0 to 8 h or with control peptide for 8 h. The genomic DNA of the bacteria was subjected to electrophoresis.