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Chimeric tRNAs are not detected in cellulo. (A) ANG-mediated cleavage site in tRNAGly-GCC-1. ANG cleaves between C and C in the anticodon of tRNAGly-GCC-1, generating 34-nt 5′-tiRNAGly-GCC-1 and 40-nt 3′-tiRNAGly-GCC-1. (B) Flowchart of the analysis to detect chimeric tRNAs comprising 5′-tRNAGly-GCC-1. For the detail, see Materials and Methods. (C) ANG treatment does not induce chimeric tRNA production in the cell.
