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Cobalamin deficiency caused an increase in SA-β-gal activity in normal human astrocytes. The cells were stained with the CellEvent Senescence Green probe. A fluorescence signal from the enzyme-cleaved product was analyzed using a confocal system. Representative microscopic images (a) and a bar graph (b) corresponding to the mean ± SD of three independent experiments were presented. p value from the unpaired t-test test is indicated. The scale bar shows 200 µm.
