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Cobalamin deficiency in astrocytes does not affect etoposide-dependent induction of apoptosis. In order to induce apoptosis, the cells were treated with 300 µM etoposide for 48 h. Then, mitochondrial membrane potential assay (a,b) and annexin V staining (c,d) were performed using image cytometry. Representative scatterplots and bar graphs (corresponding to the mean ± SD of three independent experiments were presented. p value from the unpaired t-test test is indicated).
