Effects of Latif on proliferation and apoptotic alteration in human OSCCs. (A,B) Cell viability was assessed after Latif treatment for 24 h at concentrations ranging from 0 to 100 μM in human YD-8 and YD-10B OSCCs using an MTT assay. (C) Amounts of 10, 30, and 50 μM Latif was treated for 24 h in YD-10B OSCCs, and apoptotic morphological changes were monitored under a light microscope. (D) Nuclear apoptotic DNA fragments were analyzed by TUNEL and DAPI staining and monitored under a fluorescence microscope. Scale bar: 50 μm. Data are represented as mean ± S.E.M. Significance was set at p < 0.05, indicated by an asterisk in the graph.