FIG. 1.
Purification of F. hepatica proteinases used in vaccination trials. (A) Reducing SDS-PAGE analysis, using a 15% gel, of cathepsin L-containing fractions eluted from a Sephacryl S-200 HR column (lane 1), purified 27-kDa CL1 (lane 2), and 29-kDa CL2 (lane 3). (B) Nonreducing SDS-PAGE analysis, using a 10% gel, of a detergent-soluble extract (DOC) from adult flukes (lane 1) and Bestatin affinity-purified LAP (lane 2). Shown on the right are molecular mass standards.