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. 1999 Apr;67(4):1954–1961. doi: 10.1128/iai.67.4.1954-1961.1999

FIG. 1.

FIG. 1

Purification of F. hepatica proteinases used in vaccination trials. (A) Reducing SDS-PAGE analysis, using a 15% gel, of cathepsin L-containing fractions eluted from a Sephacryl S-200 HR column (lane 1), purified 27-kDa CL1 (lane 2), and 29-kDa CL2 (lane 3). (B) Nonreducing SDS-PAGE analysis, using a 10% gel, of a detergent-soluble extract (DOC) from adult flukes (lane 1) and Bestatin affinity-purified LAP (lane 2). Shown on the right are molecular mass standards.