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. 1999 Apr;67(4):1954–1961. doi: 10.1128/iai.67.4.1954-1961.1999

FIG. 3.

FIG. 3

FIG. 3

Western blot analysis of IgG response against F. hepatica proteinases in sheep during the course of vaccination trials. (A) For the first vaccination trial, E/S products from mature flukes, separated by SDS-PAGE (12% gel) under reducing conditions and electrotransferred onto nitrocellulose filters, were probed with pools of serum obtained from animals in each group at weeks 0 (primary immunization), 6 (time of infection), and 12 (6 weeks postinfection). Sheep were immunized with 100 μg of either CL1 (group 1) or CL2 (group 2) or with PBS (group 3; control) per immunization. (B) The development of specific IgG antibodies against F. hepatica cathepsin L proteinases and LAP at various time points during trial 2 is shown. Pooled sera obtained from animals in each sheep group at weeks 0 (primary immunization), 6 (time of infection), and 12 (6 weeks postinfection) were probed against E/S products (ES) or LAP (L). Animals were immunized with 100 μg each of CL1 and CL2 (group 1), 100 μg each of CL1, CL2, and LAP (group 2), 100 μg of LAP (group 3), or PBS (group 4; control) per immunization.