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. 2022 Oct 24;23(21):12828. doi: 10.3390/ijms232112828

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cytotoxicity assays in B-ALL cell-lines comparing the cytotoxicity of parental NK-92 and CD19-CAR-NK-92 vs. irradiated, CBLB, NKG2A, TIGIT or triple knock-out cells. Specific lysis is shown as mean ± SD (n = 3). Calcein assays were incubated for 2 h and several effector-to-target (E:T) ratios were employed: 10:1, 5:1, 2.5:1. Luciferase assays were studied for a time span of 6 h at 5:1 E:T ratio. (a) NK-92 cell lines vs. KOPN-8 calcein assay. (b) CD19-CAR-NK-92 cell lines vs. KOPN-8 calcein assay. (c) NK-92 cell lines vs. MHH-CALL-4 calcein assay did not display cytotoxicity in the tested conditions (no killing). (d) CD19-CAR-NK-92 cell lines vs. MHH-CALL-4 calcein assay. (e) NK-92 cell lines vs. Nalm-6 calcein assay. (f) CD19-CAR-NK-92 cell lines vs. Nalm-6 calcein assay. (g) NK-92 cell lines vs. Nalm-6 GFP/Luc luciferase assay. (h) CD19-CAR-NK-92 cell lines vs. Nalm-6 GFP/Luc luciferase assay. * p < 0.05; ** p < 0.01; **** p < 0.0001; ns, non-significant (p > 0.05).