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Characterization of the apxIIC mutant chromosome. PCR was used to amplify the region of the A. pleuropneumoniae chromosome containing the apxIIC gene from mutant (HS93C− Ampr) and wild-type (HS93) strains. (A) PCR products were resolved on gels and stained with ethidium bromide. (B) Southern blot hybridization of the PCR products with the apxIIC gene (Tox2) and the ampicillin resistance gene (Ampr).
