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. 2022 Nov 2;23(21):13389. doi: 10.3390/ijms232113389

Figure 1.

Figure 1

Expression of the huMETCAM/MUC18 protein in the G418R-NPC-TW01 clones. HuMETCAM/MUC18 expression in the lysates prepared from the various clones/cells was determined by Western blot analysis, as described in the Section 4. The HuMETCAM/MUC18 expression level in the cell lysate of a human melanoma cell line, SK-Mel-28, was used as a positive control (lane 1) and that from the parental human nasopharyngeal cancer cell line, NPC-TW01, was used as a negative control (lane 2). The HuMETCAM/MUC18 expression in the cell lysates from three huMETCAM/MUC18 cDNA-transfected NPC-TW01 clones (clones #92, #45, and #85) and one vector control clone (#V1) are shown in lanes 3–6. The number under each lane indicates the relative level of huMETCAM/MUC18 in each cell line, assuming that that of the SK-Mel-28 was 100%. Actin is shown as the loading control.