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. 2022 Nov 2;23(21):13389. doi: 10.3390/ijms232113389

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of huMETCAM/MUC18′s over-expression on the in vitro motility (a,b) and invasiveness (c) of the NPC-TW01 cells. All in vitro motility and invasiveness tests were performed in the presence of the anti-huMETCAM/MUC18 antibody or the isotype control IgY, as described in the Section 4 The experiments were repeated three times and the means and standard deviations of triplicate values are indicated. (a,b) Effects of huMETCAM/MUC18 expression on the in vitro motility of the NPC-TW01 METCAM clone #92 and the vector control clone #V1 (a) or the NPC-TW01 METCAM clones #92 and #45 and the vector control clone #V1 (b) were determined either in the presence of anti-huMETCAM/MUC18 antibody or the control chicken IgY. The means and standard deviations of triplicate values of the motility tests are indicated. The p-values were obtained by comparing the motility of clone #92 with the control V1 clone. The p-values were also obtained by comparing the motility of the cells in the presence of the anti-huMETCAM/MUC18 antibody with that of the control IgY. (c) Effects of huMETCAM/MUC18 expression on the in vitro invasiveness of the NPC-TW01 METCAM clone #92 and the vector control clone V1 was determined either in the presence of anti-huMETCAM/MUC18 antibody or the control chicken IgY. The means and standard deviations of triplicate values of the invasiveness tests are indicated. The p-values were obtained by comparing the invasiveness of clone #92 with the control V1 clone. The p-values were also obtained by comparing the invasiveness of cells in the presence of the anti-huMETCAM/MUC18 antibody with that of the control IgY.