Figure 6.

HuMETCAM/MUC18 antigen expression in tumor lysates and in tumor tissue sections. (a) The expression of huMETCAM/MUC18 in the lysates from the tumors was determined by Western blot analysis, as described in the Section 4. The expression of huMETCAM/MUC18 in the lysates from the tissue cultured the SK-Mel-28 cells (lane 1) and the NPC-TW01 clones #92 (lane 2), #45 (lane 3), and V1 (lane 4) were used as the controls. The huMETCAM/MUC18 expression levels in the tumor lysates are shown in lanes 5–13. The huMETCAM/MUC18 expression levels in the combined lysate from the tumors of the METCAM clone #92 (lane 5), in the lysates from the tumors of the METCAM clone #45 (lanes 6–9), and in the lysates from the tumors of the vector control clone V1 (lanes 10–13) are shown. As loading controls, the same membranes were reacted with the antibody against the house-keeping gene, actin (as shown). (b) The histology and immunohistochemistry (IHC) of the tumors of the NPC-TW01 METCAM clone #92 and the vector control clone V1 are shown. Panels A and B show the histology of the tumor sections from the vector control clone V1 and panels C and D show those from clone #92. Panels E to L show the IHC of these tumor sections. A tissue section of the SC tumors derived from the human prostate cancer line LNCaP-expressing clone (LNS239) was used as a positive external control for the IHC staining (panel E). Panels E to H show the anti-huMETCAM/MUC18 antibody staining of the cells in the tumor sections and panels I to L show the negative controls without the antibody. The tumor section from the METCAM clone #92 showed strong brown color staining in the IHC when the antibody was added (panels G and H); however, the tumor section from the vector control clone V1 showed a weak background staining (panel F). Panels I to L show the corresponding negative controls, which show no staining in the adjacent sections when no antibody was added or when the control chicken IgY was added.