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. 2022 Oct 28;14(21):4549. doi: 10.3390/nu14214549

Figure 5.

Figure 5

L-cit improves the cell viability, antioxidant capacity, and Nrf2 activation in the mTEC1 cells. The mTEC1cells were exposed to 100, 150, 200, 250, and 500 μM FAC for 24 h or 48 h. Cell viability, iron concentration, LDH activity, antioxidant capacity, and Nrf2 expression were investigated. (A) Cell viability at 24 h following FAC treatment. (B) Cell viability at 48 h following FAC treatment. (C) Cell viability at 24 h following 200 μM FAC cotreated with different dosage of L-cit. (D) Cell morphology and viability treated with 2 mM L-cit, followed by 200 μM FAC for 24 h. (E) Iron concentration. (F) LDH activity. (G) MDA concentration. (H) SOD activity. (I) GSH-Px activity. (J,K) Immunoblot analyses of Nrf2 in cytoplasm. GAPDH was used as the loading control. (J,L) Immunoblot analyses of Nrf2 in nucleus. H3 (Histone H3) was used as the loading control. Values represent mean ± SEM. of three independent experiments. Significant differences are represented by * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns (not significant).