Figure 7.

L-cit suppresses ferritinophagy to ameliorate FAC-induced lipid ROS accumulation and MMP elevation in mTEC1 cells. The mTEC1 cells were transfected with 50 nM siNCOA4, followed by 2 mM L-cit, and treated with 200 μM FAC for 24 h. (A,B) Lipid ROS detection using C11 BODIPYTM^581/591 fluorescent probe. Green fluorescence indicates oxidized and red fluorescence indicates reduction. (C,D) Mitochondrial membrane potential (MMP) detection by JC-1 fluorescent probe. Green fluorescence indicates JC-1 monomer and red fluorescence indicates JC-1 aggregate. Values represent mean ± SEM. of three independent experiments. Significant differences are represented by * p < 0.05, ** p < 0.01, *** p < 0.001.