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. 2022 Oct 28;14(21):4549. doi: 10.3390/nu14214549

Figure 9.

Figure 9

L-cit restrains ferritinophagy-mediated ferroptosis to alleviate inflammation in mTEC1 cells. The mTEC1 cells were transfected with 50 nM siNCOA4, followed by 2 mM L-cit, and treated with 200 μM FAC for 24 h. Immunoblot analyses were explored. (A,B) Immunoblot analyses of TNF-α. (A,C) Immunoblot analyses of IL-6. (A,D) Immunoblot analyses of IL-1β. (A,E) Immunoblot analyses of p-p65/p65. (F) Detection of p65 nuclear translocation using immunofluorescence assay. GAPDH was used as the loading control. Values represent mean ± SEM. of three independent experiments. Significant differences are represented by * p < 0.05, ** p < 0.01, *** p < 0.001.